Description

Technical Parameters

Introduction

AOD96042

Factory 1

musclepic 6

Products Description

1. Classification and Types

From the perspective of function and purity, AOD9604 can be classified into the following categories:

Pharmaceutical Grade AOD9604: Purity is typically above 98%–99.5%, verified by HPLC (High Performance Liquid Chromatography), with extremely low impurity content. It is mainly used for clinical research, injection preparation, and pharmacological activity verification. Pharmaceutical grade products must comply with GMP standards to ensure production in a safe, pyrogen-free environment.

Research Grade AOD9604: Purity is generally between 95% and 98%, widely used in laboratory in vitro studies, cell experiments, and animal model testing. This type of product can verify the mechanisms of action of AOD9604 in lipid metabolism, energy regulation, and inflammation suppression.

Nutritional Supplement Grade AOD9604: Mostly available in lyophilized powder or formulated powder form, used in sports nutrition and anti-aging health products. This grade of AOD9604 has slightly lower purity than pharmaceutical grade, but still maintains high activity and stability. It is often diluted with solvents for subcutaneous micro-injection or transdermal absorption formulations. 2. Molecular Structure and Physicochemical Properties

The molecular formula of AOD9604 is C₇₈H₁₂₃N₂₃O₂₃S₂, with a molecular weight of approximately 1815.1 Da. It is a polypeptide segment composed of 16 amino acids, belonging to a linear peptide structure, with terminal amino and carboxyl groups, exhibiting a typical peptide bond structure. AOD9604 shares approximately 10% sequence homology with human growth hormone (HGH), but its structure is specifically optimized for the lipolysis pathway and hardly participates in the stimulation response of IGF-1 (insulin-like growth factor), therefore it will not cause bone hyperplasia or abnormal muscle growth.

2,Its main physicochemical properties are as follows:

Appearance: White or off-white lyophilized powder, loose and uniform in texture.

Solubility: Soluble in water, physiological saline, or trace amounts of acetic acid solution; stable at neutral pH, easily hydrolyzed under alkaline conditions.

pH stability range: 4.5–7.0.

Melting Point: As it is a polypeptide, it does not have a definite melting point. It is easily degraded when heated above 150℃.

Storage Conditions: Stable under refrigeration (2-8℃) or freezing (below -20℃). Avoid light exposure and repeated freeze-thaw cycles.

Color Characteristics: High-purity AOD9604 is a pure white or nearly transparent powder. If it is light yellow or grayish-white, it is often due to impurities or oxidation products.

3. Mechanism of Action and Biological Characteristics

The main function of AOD9604 is to promote lipid metabolism and inhibit lipid production. Its mechanism of action can be summarized as follows:

Promoting Lipolysis: AOD9604 activates the β-adrenergic receptor-like signaling pathway on the adipocyte membrane, increases the level of cyclic adenosine monophosphate (cAMP), and promotes the activity of hormone-sensitive lipase, thereby accelerating the breakdown of triglycerides into free fatty acids and glycerol.

Inhibiting Fat Accumulation

It can downregulate the expression of fat synthesis-related enzymes such as fatty acid synthase (FAS), and simultaneously inhibit the synthesis of new fatty acids through the AMPK (adenosine monophosphate-activated protein kinase) signaling pathway.

Promoting Fat Oxidation

Experiments have shown that AOD9604 can stimulate mitochondrial β-oxidation, increase energy metabolism rate, and enable the body's stored fat to be converted into energy more quickly.

Does Not Affect Blood Glucose and Growth Axis

Unlike traditional growth hormones, AOD9604 does not significantly affect IGF-1 levels and does not cause hyperglycemia, insulin resistance, or sodium and water retention, thus exhibiting high safety.

Assisting Repair and Anti-inflammation

Recent studies have found that AOD9604 has potential auxiliary effects in chondrocyte repair, joint damage repair, and inflammatory response regulation, and is being explored for use in osteoarthritis and sports injury rehabilitation research.

4. Main Advantages

High Safety and Low Side Effects

Because AOD9604 is derived from a natural human hormone fragment, it has high biocompatibility. Clinical studies have shown that even with long-term use, no significant adverse reactions have been observed.

Unlike HGH, AOD9604 does not stimulate the IGF-1 system, therefore it does not cause abnormal bone or muscle development, making it more suitable for long-term lipid regulation or weight control.

Significant Fat-Breaking Capacity: Experiments have shown that AOD9604 can effectively reduce abdominal, waist, and visceral fat, especially in areas that are difficult to lose fat.

Metabolic-Friendly Peptide Molecule: It has a bidirectional regulatory effect on glucose and lipid metabolism, does not affect blood sugar stability, and helps improve insulin sensitivity.

High Formulation Adaptability: Its molecular stability is superior to most short peptides, and it can be formulated into various forms such as injections, sublingual tablets, transdermal patches, and oral nanoparticles.

5. Appearance and Quality Characteristics

High-quality AOD9604 powder should be a white or off-white loose lyophilized powder with a slight sheen after absorbing moisture. Under magnification, the particles should be uniform, without visible impurities or lumps. The dissolved solution should be clear and transparent, without turbidity or sediment. If the powder is light yellow, the solution is cloudy, or has an off-odor, it indicates that the product has been oxidized or decomposed. A qualified AOD9604 should exhibit a single main peak on HPLC chromatograms, with a purity ≥98%.

6. Production Process Overview

AOD9604 is produced using solid-phase peptide synthesis (SPPS) technology, constructing peptide chains through stepwise amino acid condensation. The main process flow includes:

Grafting onto a solid-phase support and activation of amino acids;

Stepwise removal of Boc or Fmoc protecting groups and peptide bond extension;

Deprotection and cleavage under acidic conditions;

Multi-step purification (reversed-phase HPLC, high-speed centrifugation, freeze-drying).

High-purity products are packaged and stored only after quality testing. The entire process must be carried out in a sterile environment to prevent peptide chain oxidation or breakage.

7. Storage and Stability

AOD9604 has a polypeptide structure and is sensitive to temperature and humidity. Optimal storage conditions are as follows:

Unopened lyophilized powder can be stably stored for more than 12 months at temperatures below -20°C;

The dissolved solution is stable for 48 hours at 2–8°C and is recommended for immediate use;

Avoid light exposure and repeated freeze-thaw cycles to prevent peptide bond breakage or oxidation.

For long-term transportation, use a cryogenic chain storage method and encapsulate under inert gas protection.

8. Application Areas and Prospects

* **Treatment of Obesity and Metabolic Syndrome:** Clinical trials show that AOD9604 helps reduce body fat and improve insulin sensitivity, showing positive effects on patients with mild obesity and metabolic syndrome.

* **Sports and Body Shaping:** As a metabolic regulatory peptide without muscle hyperplasia side effects, AOD9604 is often used in body management, fitness shaping cycles, and exercise recovery periods.

* **Anti-aging and Repair Research:** Recent research has found that AOD9604 can promote cellular energy metabolism and improve mitochondrial function, showing potential value in delaying aging and maintaining skin firmness.

Potential for Combination Therapy

AOD9604 can synergize with peptides such as GH Frag 176-191, CJC-1295, and Ipamorelin to construct personalized lipid metabolism regulation strategies.

9. Conclusion

As an innovative metabolic regulatory peptide derived from the HGH fragment, AOD9604 successfully achieves the ideal balance of "lipid breakdown without promoting growth" thanks to its unique molecular structure and safe metabolic characteristics. High-quality AOD9604 powder not only exhibits excellent physicochemical properties (pure white, stable, and easily soluble) but also demonstrates outstanding advantages in pharmacological effects, safety, and formulation development potential. With the advancement of peptide drug technology, AOD9604 is expected to play a wider role in obesity treatment, metabolic health, and anti-aging, becoming an important representative of biopeptide products with both scientific research value and market potential.